Effects of using different biological products on IL-1β TNF-α, PGE2 concentrations in rabbits with periodontitis
DOI:
https://doi.org/10.56294/saludcyt2024.1332Keywords:
PRP, IL-1β TNF-α, PGE2, rabbits, periodontitisAbstract
Introduction: Platelet‐rich plasma (PRP) is a plasma fraction of autologous blood with a high platelet concentration, containing key cytokines and growth factors.
Methods: This study, conducted in the Clinical Surgery Department of the College of Dentistry, University of Al-Muthanna, involved 20 New Zealand white rabbits divided into four groups. A 1mm gingival wound was created on each rabbit, followed by immediate injections:
10μl saline
10μl PRP
10μl Platelet‐poor plasma (PPP)
3ng hepatocyte growth factor (HGF) in 10μl engineered tendon matrix
Rabbits were sacrificed on days 0, 1, 3, 5, and 12 for tissue harvesting.
Results: Interleukin 1 beta Increased in PPP group compared to HGF, PRP, and control groups. Tumor Necrosis Factor alpha: Increased in PPP group.While,PRP significantly reduced PGE2 levels on days 1, 3, and 5. On day 12, levels were low in both PRP and control groups. PPP slightly reduced PGE2 on days 1, 3, and 5. HGF reduced PGE2 on days 1, 3, and 12, with a transient response on day 0.
Conclusion: PRP sustains the up-regulation of proinflammatory factors like Interleukin 1 beta and Tumor Necrosis Factor alpha. Its anti-inflammatory function is partially mediated through HGF, producing similar anti-inflammatory effects.
References
1. Huang Y, Li Z, Van Dessel J, Salmon B, Huang B, Lambrichts I, et al. Effect of platelet‐rich plasma on peri‐implant trabecular bone volume and architecture: a preclinical micro‐CT study in beagle dogs. Clin Oral Implants Res. 2019;30(12):1190-9. DOI: https://doi.org/10.1111/clr.13532
2. Xu J, Gou L, Zhang P, Li H, Qiu S. Platelet‐rich plasma and regenerative dentistry. Aust Dent J. 2020;65(2):131-42. DOI: https://doi.org/10.1111/adj.12754
3.
4. Taschieri S, Lolato A, Ofer M, Testori T, Francetti L, Del Fabbro M. Immediate post-extraction implants with or without pure platelet-rich plasma: a 5-year follow-up study. Oral Maxillofac Surg. 2017;21(2):147-57. DOI: https://doi.org/10.1007/s10006-017-0609-2
5. Martínez CE, González SA, Palma V, Smith PC. Platelet-poor and platelet-rich plasma stimulate bone lineage differentiation in periodontal ligament stem cells. J Periodontol. 2016;87(2):e18-26. DOI: https://doi.org/10.1902/jop.2015.150360
6. Shahidi M, Vatanmakanian M, Arami MK, Shirazi FS, Esmaeili N, Hydarporian S, et al. A comparative study between platelet-rich plasma and platelet-poor plasma effects on angiogenesis. Med Mol Morphol. 2018;51(1):21-31. DOI: https://doi.org/10.1007/s00795-017-0168-5
7. Zheng C, Zhu Q, Liu X, Huang X, He C, Jiang L, et al. Improved peripheral nerve regeneration using acellular nerve allografts loaded with platelet-rich plasma. Tissue Eng Part A. 2014;20(23-24):3228-40. DOI: https://doi.org/10.1089/ten.tea.2013.0729
8. Creaney L, Hamilton B. Growth factor delivery methods in the management of sports injuries: the state of play. Br J Sports Med. 2008;42(5):314-20. DOI: https://doi.org/10.1136/bjsm.2007.040071
9. Rowe WB. Principles of modern grinding technology. William Andrew; 2013. DOI: https://doi.org/10.1016/B978-0-323-24271-4.00016-6
10. Dhurat R, Sukesh MS. Principles and methods of preparation of platelet-rich plasma: a review and author's perspective. J Cutan Aesthet Surg. 2014;7(4):189. DOI: https://doi.org/10.4103/0974-2077.150734
11. Kostić I, Mihailović D, Najman S, Stojanović S, Kostić M. The rabbit gingival tissue response to retraction liquids and tetrahydrozoline. Vojnosanit Pregl. 2014;71(1):46-51. DOI: https://doi.org/10.2298/VSP1401046K
12. Zhang J, Middleton KK, Fu FH, Im HJ, Wang JH. HGF mediates the anti-inflammatory effects of PRP on injured tendons. PLoS One. 2013;8(6):e67303. DOI: https://doi.org/10.1371/journal.pone.0067303
13. Mishra P, Pandey CM, Singh U, Gupta A, Sahu C, Keshri A. Descriptive statistics and normality tests for statistical data. Ann Card Anaesth. 2019;22(1):67. DOI: https://doi.org/10.4103/aca.ACA_157_18
14. Lin PH, Hirko MK, Von Fraunhofer JA, Greisler HP. Wound healing and inflammatory response to biomaterials. In: Wound closure biomaterials and devices. CRC Press; 2018. p. 7-24. DOI: https://doi.org/10.1201/9780203733653-2
15. Qing C. The molecular biology in wound healing & non-healing wound. Chin J Traumatol. 2017;20(4):189-93. DOI: https://doi.org/10.1016/j.cjtee.2017.06.001
16. Li Y, Wang G, Liu J, Ouyang L. Quinolizidine alkaloids derivatives from Sophora alopecuroides Linn: Bioactivities, structure-activity relationships and preliminary molecular mechanisms. Eur J Med Chem. 2020; 188:111972. DOI: https://doi.org/10.1016/j.ejmech.2019.111972
17. Tavassoli-Hojjati S, Sattari M, Ghasemi T, Ahmadi R, Mashayekhi A. Effect of platelet-rich plasma concentrations on the proliferation of periodontal cells: an in vitro study. Eur J Dent. 2016;10(4):469-74. DOI: https://doi.org/10.4103/1305-7456.195165
18. Andia I, Martin JI, Maffulli N. Advances with platelet rich plasma therapies for tendon regeneration. Expert Opin Biol Ther. 2018;18(4):389-98. DOI: https://doi.org/10.1080/14712598.2018.1424626
19. Lee MJ, Yoon KS, Oh S, Shin S, Jo CH. Allogenic Pure Platelet-Rich Plasma Therapy for Adhesive Capsulitis: A Bed-to-Bench Study With Propensity Score Matching Using a Corticosteroid Control Group. Am J Sports Med. 2021;49(9):2309-20. DOI: https://doi.org/10.1177/03635465211018636
20. Liebig BE, Kisiday JD, Bahney CS, Ehrhart NP, Goodrich LR. The platelet‐rich plasma and mesenchymal stem cell milieu: A review of therapeutic effects on bone healing. J Orthop Res. 2020;38(12):2539-50. DOI: https://doi.org/10.1002/jor.24786
21. Moussa M, Lajeunesse D, Hilal G, El Atat O, Haykal G, Serhal R, et al. Platelet rich plasma (PRP) induces chondroprotection via increasing autophagy, anti-inflammatory markers, and decreasing apoptosis in human osteoarthritic cartilage. Exp Cell Res. 2017;352(1):146-56. DOI: https://doi.org/10.1016/j.yexcr.2017.02.012
22.
23. Blandford SN, Galloway DA, Williams JB, Arsenault S, Brown J, MacLean G, et al. Interleukin-1 receptor antagonist: An exploratory plasma biomarker that correlates with disability and provides pathophysiological insights in relapsing-remitting multiple sclerosis. Mult Scler Relat Disord. 2021;52:103006. DOI: https://doi.org/10.1016/j.msard.2021.103006
24. Lu J, Ren B, Wang L, Li M, Liu Y. Preparation and evaluation of IL-1ra-loaded dextran/PLGA microspheres for inhibiting periodontal inflammation in vitro. Inflammation. 2020;43(1):168-78. DOI: https://doi.org/10.1007/s10753-019-01107-w
25. Zhao R, Wang S, Jia L, Li Q, Qiao J, Peng X. Interleukin-1 receptor antagonist protein (IL-1Ra) and miR-140 overexpression via pNNS-conjugated chitosan-mediated gene transfer enhances the repair of full-thickness cartilage defects in a rabbit model. Bone Joint Res. 2019;8(3):165-78. DOI: https://doi.org/10.1302/2046-3758.83.BJR-2018-0222.R1
26. Lopes AH, Brandolini L, Aramini A, Bianchini G, Silva RL, Zaperlon AC, et al. DF2755A, a novel non-competitive allosteric inhibitor of CXCR1/2, reduces inflammatory and post-operative pain. Pharmacol Res. 2016;103:69-79. DOI: https://doi.org/10.1016/j.phrs.2015.11.005
27. Cook AE, Cook JL, Stoker AM. Metabolic responses of meniscus to IL-1β. J Knee Surg. 2018;31(9):834-40. DOI: https://doi.org/10.1055/s-0037-1615821
28. Oryan A, Alidadi S, Moshiri A. Platelet-rich plasma for bone healing and regeneration. Expert Opin Biol Ther. 2016;16(2):213-32. DOI: https://doi.org/10.1517/14712598.2016.1118458
29. Toldo S, Abbate A. The NLRP3 inflammasome in acute myocardial infarction. Nat Rev Cardiol. 2018;15(4):203-14. DOI: https://doi.org/10.1038/nrcardio.2017.161
30. Sanchez M, Delgado D, Sanchez P, Muinos-Lopez E, Paiva B, Granero-Molto F, et al. Combination of intra-articular and intraosseous injections of platelet rich plasma for severe knee osteoarthritis: a pilot study. Biomed Res Int. 2016. DOI: https://doi.org/10.1155/2016/4868613
31. Tang C, Chen Y, Huang J, Zhao K, Chen X, Yin Z, et al. The roles of inflammatory mediators and immunocytes in tendinopathy. J Orthop Translat. 2018;14:23-33. DOI: https://doi.org/10.1016/j.jot.2018.03.003
32. Vadalà G, Ambrosio L, Russo F, Papalia R, Denaro V. Interaction between mesenchymal stem cells and intervertebral disc microenvironment: from cell therapy to tissue engineering. Stem Cells Int. 2019. DOI: https://doi.org/10.1155/2019/2376172
33. Lisowska B, Kosson D, Domaracka K. Lights and shadows of NSAIDs in bone healing: the role of prostaglandins in bone metabolism. Drug Des Devel Ther. 2018;12:1753-65. DOI: https://doi.org/10.2147/DDDT.S164562
Published
Issue
Section
License
Copyright (c) 2024 Samer Salim Jaafer, Faten Ben Amor (Author)
This work is licensed under a Creative Commons Attribution 4.0 International License.
The article is distributed under the Creative Commons Attribution 4.0 License. Unless otherwise stated, associated published material is distributed under the same licence.
