Comparison of Detection of HPV DNA from Menstrual Blood in Menstrual Cup and Cervical Swab in Sexual Single Partner and Multi-Partner Women

Authors

DOI:

https://doi.org/10.56294/saludcyt20241291

Keywords:

HPV DNA, Menstrual Blood, Cervical Swab, Women With Multiple Sexual Partners, Menstrual Cup

Abstract

Introduction: Human Papillomavirus (HPV) is a virus found in the cervix of a sexually active woman. HPV enters micro lesions in the cervical epithelium binds to primary receptors in the membrane layer over time and becomes an invasive cancer. The invasive cancer process takes 5-10 years. Detection of HPV DNA has currently used cervical swab samples. Currently, HPV DNA can be detected in menstrual blood. 
Objective: this study aimed to analyze the comparison of HPV DNA from menstrual blood in menstrual cups and cervical swabs and to analyze the risk factors associated with positive HPV DNA in single-partner and multi-partner women.
Method: cross-sectional method at one time with an analytical observational method using consecutive sampling, sample selection according to the researcher's criteria. The sample used was 44 women according to the inclusion and exclusion criteria. The Spearman statistical test was used to determine the comparison of results from detecting HPV DNA from menstrual blood and cervical swabs, determining the analysis of risk factors (age, occupation, parity, sexual partners, contraception, and smoking) related to positive HPV DNA in both groups using the chi-square test. and Spearman test according to the data scale. 
Results: there was no difference in results between HPV DNA from menstrual blood in menstrual cups and cervical swabs in both the single-partner and multi-sexual partner groups of women with p= 0,209 and 0,301. 
Conclusion: there is a comparison of HPV DNA detection from menstrual blood in menstrual cups and cervical swabs in single-partner and multi-sexual partner women. The accuracy of menstrual blood examination results is good in detecting HPV DNA

References

1. Wilailak S, Kengsakul M, Kehoe S. Worldwide initiatives to eliminate cervical cancer. Int J Gynecol Obstet. 2021;155(S1):102–6.

2. Khan TM, Buksh MA, Rehman IU, Saleem A. Knowledge, attitudes, and perception towards human papillomavirus among university students in Pakistan. Papillomavirus Res. 2016;2:122–7.

3. Ficht AL, Lapidos-Salaiz I, Phelps BR. Eliminating cervical cancer: Promising developments in primary prevention. Cancer. 2020;126(2):242–6.

4. Condrat CE, Cretoiu D, Radoi VE, Mihele DM, Tovaru M, Bordea CI, et al. Unraveling Immunological Dynamics: HPV Infection in Women—Insights from Pregnancy. Viruses. 2023;15(10):1–25.

5. Milano G, Guarducci G, Nante N, Montomoli E, Manini I. Human Papillomavirus Epidemiology and Prevention: Is There Still a Gender Gap? Vaccines. 2023;11(6).

6. Chakravarti P, Maheshwari A, Tahlan S, Kadam P, Bagal S, Gore S, et al. Diagnostic accuracy of menstrual blood for human papillomavirus detection in cervical cancer screening: a systematic review. Ecancermedicalscience. 2022;16:1–13.

7. Warashinta D, Astari A, Merdikawati A. Analysis of the Use of Menstrual Pad, Tampons, and Menstrual Cup During Menarche. J Community Heal Prev Med. 2021;1(2):24–31.

8. Gharacheh M, Ranjbar F, Hajinasab N, Haghani S. Acceptability and safety of the menstrual cups among Iranian women: a cross-sectional study. BMC Womens Health. 2021;21(1):1–8.

9. Dr. Nadia El Borai, Masato Inoue, Christophe Lefèvre, Elena N. Naumova, Bunzo Sato MYT determine a possible link between herpes simplex virus 1 (HSV) and infertility., Method: A specifically designed polymerase chain reaction with nested primers was developed and used to test for H in 153 men and 20 women attending an infertility clinic., Results: HSV DNA was detected in 37 (24%) out of 153 semen samples and in 11 (55%) out of 20 menstrual blood samples. However HD (0%) was not detected in the semen of 16 males with children., A significant association between. Detection of Herpes Simplex DNA in Semen and Menstrual Blood of Individuals Attending an Infertility ClinicNo Title. J Obstet Gynaecol Res. 2010;

10. Cvetković Vega A, Maguiña JL, Soto A, Lama-Valdivia J, Correa López LE. Cross-sectional studies. Rev la Fac Med Humana. 2021;21(1):164–70.

11. Nguyen HDT, Le TM, Lee E, Lee D, Choi Y, Cho J, et al. Relationship between Human Papillomavirus Status and the Cervicovaginal Microbiome in Cervical Cancer. Microorganisms. 2023;11(6):1–16.

12. Poljak M, Cuschieri K, Alemany L, Vorsters A. Testing for Human Papillomaviruses in Urine, Blood, and Oral Specimens: an Update for the Laboratory. J Clin Microbiol. 2023;61(8).

13. Sefuthi T, Nkonki L. A systematic review of economic evaluations of cervical cancer screening methods. Syst Rev. 2022;11(1):1–16.

14. Derbie A, Mekonnen D, Nibret E, Maier M, Woldeamanuel Y, Abebe T. Human papillomavirus genotype distribution in Ethiopia: an updated systematic review. Virol J. 2022;19(1):4–11.

15. Farahmand M, Moghoofei M, Dorost A, Abbasi S, Monavari SH, Kiani SJ, et al. Prevalence and genotype distribution of genital human papillomavirus infection in female sex workers in the world: A systematic review and meta-analysis. BMC Public Health. 2020;20(1):1–14.

16. Kops NL, Caierão J, Bessel M, Horvath JDC, Domingues CM, Benzaken AS, et al. Behavioral factors associated with multiple-type HPV genital infections: data from a cross-sectional study in young women in Brazil. Reprod Health. 2021;18(1):1–9.

17. Wong SCC, Au TCC, Chan SCS, Ng LPW, Tsang HF. Menstrual blood human papillomavirus DNA and TAP1 gene polymorphisms as potential biomarkers for screening and monitoring of cervical squamous intraepithelial lesion. J Infect Dis. 2018;218(11):1739–45.

18. Tsang HF, Cheung YS, Yu CSA, Chan CSS, Wong CBT, Yim KYA, et al. Menstrual Blood as a Diagnostic Specimen for Human Papillomavirus Genotyping and Genital Tract Infection Using Next-Generation Sequencing as a Novel Diagnostic Tool. Diagnostics. 2024;14(7).

19. Huang Y, Wu X, Lin Y, Li W, Liu J, Song B. Multiple sexual partners and vaginal microecological disorder are associated with HPV infection and cervical carcinoma development. Oncol Lett. 2020;20(2):1915–21.

20. Xu X, Zhang Y, Yu L, Shi X, Min M, Xiong L, et al. A cross-sectional analysis about bacterial vaginosis, high-risk human papillomavirus infection, and cervical intraepithelial neoplasia in Chinese women. Sci Rep. 2022;12(1):1–12.

21. Leinonen MK, Anttila A, Malila N, Dillner J, Forslund O, Nieminen P. Type- and age-specific distribution of human papillomavirus in women attending cervical cancer screening in Finland. Br J Cancer. 2013;109(11):2941–50.

22. Lee B, Cho HY, Jeon KJ, Kim K, Lee JR, Moon JJ, et al. Detection of high-risk human papillomavirus using menstrual blood in women with high-grade squamous intraepithelial lesions or high-risk human papillomavirus infections: A pilot study. J Obstet Gynaecol Res. 2016;42(3):319–24.

23. Naseri S, Young S, Cruz G, Blumenthal PD. Screening for High-Risk Human Papillomavirus Using Passive, Self-Collected Menstrual Blood. Obstet Gynecol. 2022;140(3):470–6.

24. Wong SCC, Au TCC, Chan SCS, Chan CML, Lam MYY, Zee BCY, et al. Human papillomavirus DNA detection in menstrual blood from patients with cervical intraepithelial neoplasia and condyloma acuminatum. J Clin Microbiol. 2018;48(3):709–13.

25. Yang J, Wang W, Wang Z, Wang Z, Wang Y, Wang J, et al. Prevalence, genotype distribution and risk factors of cervical HPV infection in Yangqu, China: a population-based survey of 10086 women. Hum Vaccines Immunother. 2020;16(7):1645–52.

26. Liu Y, Ang Q, Wu H, Xu J, Chen D, Zhao H, et al. Prevalence of human papillomavirus genotypes and precancerous cervical lesions in a screening population in Beijing, China: Analysis of results from China's top 3 hospitals, 2009-2019. Virol J. 2020;17(1):1–10.

27. Tekalegn Y, Sahiledengle B, Woldeyohannes D, Atlaw D, Degno S, Desta F, et al. High parity is associated with increased risk of cervical cancer: Systematic review and meta-analysis of case-control studies. Women's Heal. 2022;18.

28. Gadducci A, Cosio S, Fruzzetti F. Estro-progestin contraceptives and risk of cervical cancer: A debated issue. Anticancer Res. 2020;40(11):5995–6002.

29. Mansour MBL, Crone MR, Sert E, Van Weert HC, Chavannes NH, Van Asselt KM. Smoking cessation strategy in the national cervical cancer screening program (SUCCESS): study protocol for a pragmatic cluster randomized trial and process evaluation in Dutch general practice. BMJ Open. 2022;12(4):1–16.

30. Ono A, Nakagawa M, Ikuta E, Watanabe Y, Koshiyama M. Relationship between Tobacco Smoking and Cervical Cancer. Women’s Heal – Open J. 2019;5(1):19–21.

31. Kum-Nji H, Meloy L, Keyser-Marcus L. Tobacco smoke exposure as a risk factor for human papillomavirus infections in women 18-26 years old in the United States. PLoS One. 2019;14(10):1–9.

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Published

2024-06-02

How to Cite

1.
Suryani Arifin E, Mulawardhana P, Wardhani P. Comparison of Detection of HPV DNA from Menstrual Blood in Menstrual Cup and Cervical Swab in Sexual Single Partner and Multi-Partner Women. Salud, Ciencia y Tecnología [Internet]. 2024 Jun. 2 [cited 2024 Dec. 4];4:1291. Available from: https://sct.ageditor.ar/index.php/sct/article/view/669