Investigation of oxidation resistance of ascorbic acid in gamma-ray-induced solid samples
DOI:
https://doi.org/10.56294/saludcyt20262606Keywords:
DPPH, kinectics, antioxidant activity, Ascorbic acid, Gamma, Radiation sourceAbstract
Introduction: This study investigated the effects of gamma irradiation on the antioxidant activity of solid-state ascorbic acid. The goal was to assess how different irradiation doses, ranging from 0 to 5 kGy, altered the molecule's ability to scavenge free radicals.
Methods: The DPPH radical scavenging method was employed to determine the antioxidant capability of the irradiated samples. The researchers evaluated the reaction rate constant (K1) and the concentration required to achieve 50 % inhibition of the radicals (IC50). Furthermore, FT-IR spectroscopy was used to detect structural changes in the ascorbic acid molecule following irradiation.
Results: Antioxidant activity significantly increased at low irradiation doses (1–2 kGy) compared to the control (0 kGy, IC50 of 3,39 ± 5,73 µg/mL). The lowest IC50 values, indicating maximum activity, were observed at 1 kGy (2,67 ± 1,75 µg/mL) and 2 kGy (2,55 ±1,00 µg/mL). The reaction rate constant peaked at 1 kGy (13,78 M-1.s-1), confirming the enhanced radical scavenging capacity. However, activity decreased at higher doses (≥ 3kGy). FT-IR analysis indicated structural alterations, specifically in the hydroxyl (-OH) and carbonyl (C=O) groups, following the irradiation process.
Conclusions: The results demonstrated that appropriate levels of gamma irradiation can increase the antioxidant effectiveness of ascorbic acid. Conversely, excessive exposure leads to structural changes and a subsequent reduction in its effectiveness.
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Copyright (c) 2026 Vuong Van Loc, Tran Thi Nhan, Nguyen Manh Ha, Tran Minh Quynh, Nguyen Thuy Linh, Nguyen Thị Dung (Author)
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