Prevention of Mutagenesis, Oxidative Stress and Inflammation in first Generation Male Rats whose Parents are Exposed to Gamma Radiation and Hexavalent Chromium
DOI:
https://doi.org/10.56294/saludcyt20251259Keywords:
Radiation Exposure, Hexavalent Chromium, Oxidative Stress, Genomic Instability, Stochastic Pathologies, Burdock Root OilAbstract
Introduction: Children exposed to radiation chemical agents or born to exposed parents faced elevated risks of stochastic pathologies, including genetic disorders, tumours, and leukaemia. These risks were attributed to mutations and latent genomic damage caused by such exposures.
Methods: This six-month experimental study at NAO ZKMU in Kazakhstan evaluated Burdock Root Oil's protective effects against gamma radiation and hexavalent chromium-induced damage in 100 Wistar rats, analyzed using Statistica 10.
Results: The findings revealed that the offspring of parents exposed to combined chromium and gamma irradiation (Cr⁶⁺ + γ) exhibited a 33% increase in micronuclei (6.3 ±1.16‰, P ≤ 0.01) compared to controls (4.56 ± 1.18‰), indicating significant genotoxicity. Burdock Root Oil reduced micronuclei levels to 5.34 ± 0.792‰ (P≥ 0.05), comparable to control levels. Chromosomal aberrations in the Cr⁶⁺+ γgroup increased by 54% (2.77 ± 0.537%, P ≤ 0.001), while Burdock Root Oil reduced total aberrations by 19.5% (P ≤ 0.005). Markers of oxidative stress showed significant improvement; superoxide dismutase (SOD) activity increased by 16.7%, catalase by 22.6%, and sulfhydryl groups by 23% (P≤ 0.05), while malondialdehyde levels decreased by 16% (P ≤ 0.05). The SH/MDA ratio increased by 45% (P ≤ 0.05).
Conclusions: Burdock Root Oil effectively mitigated genotoxic, oxidative, and inflammatory effects in the offspring of parents exposed to gamma radiation and chromium. It restored immune balance, reduced oxidative stress, and preserved genomic stability
References
1. Stephens J, Moorhouse AJ, Craenen K, Schroeder E, Drenos F, Anderson R. A systematic review of human evidence for the intergenerational effects of exposure to ionizing radiation. Int J Radiat Biol [Internet]. 2024 Feb 09 [cited 2024 Nov 11];100(9):1330–63. Available from: https://www.tandfonline.com/doi/abs/10.1080/09553002.2024.2306328
2. Benotmane MA, Trott KR. Epidemiological and experimental evidence for radiation-induced health effects in the progeny after exposure in utero. Int J Radiat Biol [Internet]. 2024 Dec 11 [cited 2024 Nov 11];100(9):1264–75. Available from: https://www.tandfonline.com/doi/abs/10.1080/09553002.2023.2283088
3. Amrenova A, Baudin C, Ostroumova E, Stephens J, Anderson R, Laurier D. Intergenerational effects of ionizing radiation: review of recent studies from human data (2018–2021). Int J Radiat Biol [Internet]. 2024 Feb 06 [cited 2024 Nov 11];100(9):1253–63. Available from: https://www.tandfonline.com/doi/abs/10.1080/09553002.2024.2309917
4. Reindl J, Abrantes AM, Ahire V, Azimzadeh O, Baatout S, Baeyens A, et al. Molecular Radiation Biology. Radiobiology Textbook [Internet]. 2023 Jan 1 [cited 2024 Nov 11];83–189. Available from: https://link.springer.com/chapter/10.1007/978-3-031-18810-7_3
5. Dong S, Lyu X, Yuan S, Wang S, Li W, Chen Z, et al. Oxidative stress: A critical hint in ionizing radiation induced pyroptosis. Radiat Med Prot [Internet]. 2020 Dec 1 [cited 2024 Nov 11];1(4):179–85. Available from: https://mednexus.org/doi/full/10.1016/j.radmp.2020.10.001
6. El Adham EK, Hassan AI, A. Dawoud MM. RETRACTED ARTICLE: Evaluating the role of propolis and bee venom on the oxidative stress induced by gamma rays in rats. Scientific Reports 2022 12:1 [Internet]. 2022 Feb 16 [cited 2024 Nov 11];12(1):1–22. Available from: https://www.nature.com/articles/s41598-022-05979-1
7. Mohammadgholi M, Hosseinimehr SJ. Crosstalk between Oxidative Stress and Inflammation Induced by Ionizing Radiation in Healthy and Cancerous Cells. Curr Med Chem [Internet]. 2024 Apr 7 [cited 2024 Nov 11];31(19):2751–69. Available from: https://pubmed.ncbi.nlm.nih.gov/37026495/
8. Alkis H, Demir E, Taysi MR, Sagir S, Taysi S. Effects of Nigella sativa oil and thymoquinone on radiation-induced oxidative stress in kidney tissue of rats. Biomedicine & Pharmacotherapy [Internet]. 2021 Jul 1 [cited 2024 Nov 11]; 139:111540. Available from: https://doi.org/10.1016/j.biopha.2021.111540
9. Xie LW, Cai S, Zhao TS, Li M, Tian Y. Green tea derivative (−)-epigallocatechin-3-gallate (EGCG) confers protection against ionizing radiation-induced intestinal epithelial cell death both in vitro and in vivo. Free Radic Biol Med [Internet]. 2020 Dec 1 [cited 2024 Nov 11]; 161:175–86. Available from: https://doi.org/10.1016/j.freeradbiomed.2020.10.012
10. Mothersill C, Rusin A, Elliott A, Seymour C. Radiation and chemical induced genomic instability as a driver for environmental evolution. Genome Stability: From Virus to Human Application [Internet]. 2021 Jan 1 [cited 2024 Nov 11];639–58. Available from: https://doi.org/10.1016/B978-0-323-85679-9.00034-9
11. Sosnina SF, Kabirova NR, Sokolnikov ME, Okatenko P V. The risk of oncohematological pathology in children of workers employed at radiation hazardous production. Health Risk Anal [Internet]. 2019 Mar 30 [cited 2024 Nov 11];(1):30–9. Available from: https://journal.fcrisk.ru/eng/2019/1/3
12. Bazyka D, Hatch M, Gudzenko N, Cahoon EK, Drozdovitch V, Little MP, et al. Field Study of the Possible Effect of Parental Irradiation on the Germline of Children Born to Cleanup Workers and Evacuees of the Chornobyl Nuclear Accident. Am J Epidemiol [Internet]. 2020 Dec 1 [cited 2024 Nov 11];189(12):1451–60. Available from: https://dx.doi.org/10.1093/aje/kwaa095
13. Coretchi L, Gincu M, Bahnarel I, Friptuleac G, Romanciuc P, Capatina A. Clinical, immunological and genetic research on the participants in mitigating the consequences of the Chernobyl nuclear accident. OHRAD [Internet]. 2023 Dec 28 [cited 2024 Nov 11];4(1):4–19. Available from: https://doi.org/10.38045/ohrm.2023.1.01
14. Skinner MK, Nilsson EE. Role of environmentally induced epigenetic transgenerational inheritance in evolutionary biology: Unified Evolution Theory. Environ Epigenet [Internet]. 2021 Nov 26 [cited 2024 Nov 11];7(1). Available from: https://dx.doi.org/10.1093/eep/dvab012
15. Jit BP, Pattnaik S, Arya R, Dash R, Sahoo SS, Pradhan B, et al. Phytochemicals: A potential next generation agent for radioprotection. Phytomedicine [Internet]. 2022 Nov 1 [cited 2024 Nov 11]; 106:154188. Available from: https://doi.org/10.1016/j.phymed.2022.154188
16. Bakloushinskaya I. Chromosome Changes in Soma and Germ Line: Heritability and Evolutionary Outcome. Genes 2022 [Internet]. 2022 Mar 28 [cited 2024 Nov 11];13(4):602. Available from: https://doi.org/10.3390/genes13040602
17. Abdulameer JH, Obaid NH, Manshad MJ. A review: The Impact of Environmental Heavy Metal Contamination on Human Health. PSIJK [Internet]. 2024 Sep 30 [cited 2024 Nov 11];1(3):17–25. Available from: https://journals.uokerbala.edu.iq:8443/index.php/psijk/article/view/1467
18. Iztleuov M, Kaliev A, Turganbaeva A, Yesmukhanova D, Akhmetova A, Temirbayeva A, et al. The effect of sodium tetraborate on chromium-induced oxidative damages in rats lung tissue. Biomed Pharmacol J [Internet]. 2020 Mar 13 [cited 2024 Nov 11];13(1):281–90. Available from: https://dx.doi.org/10.13005/bpj/1887
19. Jiang Z, Schenk L, Assarsson E, Albin M, Bertilsson H, Dock E, et al. Hexavalent chromium still a concern in Sweden – Evidence from a cross-sectional study within the SafeChrom project. Int J Hyg Environ Health [Internet]. 2024 Mar 1 [cited 2024 Nov 11];256:114298. Available from: https://doi.org/10.1016/j.ijheh.2023.114298
20. Thangagiri B, Sivakumar R. Biochar for toxic chromium removal: Its impacts, mechanism, and future direction. Biomass Conv Bioref [Internet]. 2023 Mar 25 [cited 2024 Nov 11];14(16):18417–44. Available from: https://link.springer.com/article/10.1007/s13399-023-04058-3
21. Abdullah, Wani KI, Naeem M, Jha PK, Jha UC, Aftab T, et al. Systems biology of chromium-plant interaction: insights from omics approaches. Front Plant Sci [Internet]. 2023 Jan 8 [cited 2024 Nov 11];14:1305179. Available from: https://doi.org/10.3389/fpls.2023.1305179
22. Ma B, Al-Wraikat M, Shu Q, Yang X, Liu Y. An Overview of Interactions between Goat Milk Casein and Other Food Components: Polysaccharides, Polyphenols, and Metal Ions. Foods [Internet]. 2024 Sep 13 [cited 2024 Nov 11];13(18):2903. Available from: https://www.mdpi.com/2304-8158/13/18/2903/htm
23. Lou J, Zhou Y, Feng Z, Ma M, Yao Y, Wang Y, et al. Caspase-Independent Regulated Necrosis Pathways as Potential Targets in Cancer Management. Front Oncol [Internet]. 2021 Feb 16 [cited 2024 Nov 11];10:616952. Available from: https://doi.org/10.3389/fonc.2020.616952
24. Baig S. Change in physical and mental health due to aging: future perspective. Futur Med [Internet]. 2023 Mar 30 [cited 2024 Nov 11];2(1):13-2. Available from: https://futurity-medicine.com/index.php/fm/article/view/22
25. Talapko J, Talapko D, Katalinić D, Kotris I, Erić I, Belić D, et al. Health Effects of Ionizing Radiation on the Human Body. Medicina [Internet]. 2024 Apr 18 [cited 2024 Nov 11];60(4):653. Available from: https://www.mdpi.com/1648-9144/60/4/653/htm
26. Stasiłowicz-Krzemień A, Gościniak A, Formanowicz D, Cielecka-Piontek J. Natural Guardians: Natural Compounds as Radioprotectors in Cancer Therapy. Int J Mol Sci [Internet]. 2024 Jun 25 [cited 2024 Nov 11];25(13):6937. Available from: https://www.mdpi.com/1422-0067/25/13/6937/htm
27. Chaudhary P, Janmeda P, Docea AO, Yeskaliyeva B, Abdull Razis AF, Modu B, et al. Oxidative stress, free radicals and antioxidants: potential crosstalk in the pathophysiology of human diseases. Front Chem [Internet]. 2023 May 10 [cited 2024 Nov 11];11:1158198. Available from: https://doi.org/10.3389/fchem.2023.1158198
28. Iztleuov M, Iztleuov Y, Saparbayev S, Temirbayeva A, Medeuova R, Aleuova Z, et al. Effect of Burdock Root Oil on Oxidative Stress Induced by Isolated and Combined Use of Gamma Radiation and Hexavalent Chromium. Biomed Pharmacol J [Internet]. 2022 Mar 29 [cited 2024 Nov 11];15(1):421–32. Available from: https://dx.doi.org/10.13005/bpj/2382
29. Bjørklund G, Cruz-Martins N, Goh BH, Mykhailenko O, Lysiuk R, Shanaida M, et al. Medicinal Plant-derived Phytochemicals in Detoxification. Curr Pharm Des [Internet]. 2023 Aug 10 [cited 2024 Nov 11];30(13):988–1015. Available from: https://www.benthamdirect.com/content/journals/cpd/10.2174/1381612829666230809094242
30. Shyam M, Sabina EP. Harnessing the power of Arctium lappa root: a review of its pharmacological properties and therapeutic applications. Nat Prod Bioprospect [Internet]. 2024 Dec 1 [cited 2024 Nov 11];14(1). Available from: https://doi.org/10.1007/s13659-024-00466-8
31. Cedillo-Cortezano M, Martinez-Cuevas LR, López JAM, Barrera López IL, Escutia-Perez S, Petricevich VL. Use of Medicinal Plants in the Process of Wound Healing: A Literature Review. Pharmaceuticals [Internet]. 2024 Feb 27 [cited 2024 Nov 11];17(3):303. Available from: https://www.mdpi.com/1424-8247/17/3/303/htm
32. Arifin WN, Zahiruddin WM. Sample Size Calculation in Animal Studies Using Resource Equation Approach. Malays J Med Sci [Internet]. 2017 Oct 26 [cited 2024 Nov 11];24(5):101–5. Available from: https://europepmc.org/articles/PMC5772820
33. Otani K, Ohtaki M, Fujimoto N, Saimova A, Chaizhunusova N, Rakhypbekov T, et al. Quantitative Analysis of Effects of a Single 60Co Gamma Ray Point Exposure on Time-Dependent Change in Locomotor Activity in Rats. Int J Environ Res Public Health [Internet]. 2020 Aug 2 [cited 2024 Nov 11];17(16):5638. Available from: https://pmc.ncbi.nlm.nih.gov/articles/PMC7459625/
34. Russo MT, De Luca G, Palma N, Leopardi P, Degan P, Cinelli S, et al. Oxidative Stress, Mutations and Chromosomal Aberrations Induced by In Vitro and In Vivo Exposure to Furan. Int J Mol Sci [Internet]. 2021 Sep 7 [cited 2024 Nov 11];22(18):9687. Available from: https://www.mdpi.com/1422-0067/22/18/9687/htm
35. Gordeev II, Mikryakov D V., Silkina NI, Mikryakov VR, Busarova OYu. Oxidation Processes and the Content of Immune Complexes in Immunocompetent Organs of Char in the Basin of Kronotskoe Lake. Biol Bull Russ Acad Sci [Internet]. 2022 Mar 29 [cited 2024 Nov 11];48(3):S136–40. Available from: https://link.springer.com/article/10.1134/S1062359022010071
36. Cicconi F, Ubaldini A, Fiore A, Rizzo A, Cataldo S, Agostini P, et al. Dissolution of Molybdenum in Hydrogen Peroxide: A Thermodynamic, Kinetic and Microscopic Study of a Green Process for 99mTc Production. Molecules [Internet]. 2023 Mar 1 [cited 2024 Nov 11];28(5):2090. Available from: https://pmc.ncbi.nlm.nih.gov/articles/PMC10004273/
37. Andrés CMC, Pérez de la Lastra JM, Andrés Juan C, Plou FJ, Pérez-Lebeña E. Superoxide Anion Chemistry—Its Role at the Core of the Innate Immunity. Int J Mol Sci [Internet]. 2023 Jan 17 [cited 2024 Nov 11];24(3):1841. Available from: https://www.mdpi.com/1422-0067/24/3/1841/htm
38. Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys [Internet]. 1959 [cited 2024 Nov 8];82(1):70–7. Available from: https://pubmed.ncbi.nlm.nih.gov/13650640/
39. Human TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit - CD Biosynsis [Internet]. [cited 2024 Nov 8]. Available from: https://www.biosynsis.com/human-tnf-tumor-necrosis-factor-alpha-elisa-kit-item-22017.html/
40. Codecasa E, Pageat P, Marcet-Rius M, Cozzi A. Legal Frameworks and Controls for the Protection of Research Animals: A Focus on the Animal Welfare Body with a French Case Study. Animals [Internet]. 2021 Mar 5 [cited 2024 Nov 11];11(3):695. Available from: https://www.mdpi.com/2076-2615/11/3/695/htm
41. Ausems EJ. The european convention for the protection of vertebrate animals used for experimental and other scientific purposes. Z Fur Vers. 1986;28:219.
42. Little MP, Goodhead DT, Bridges BA, Bouffler SD. Evidence relevant to untargeted and transgenerational effects in the offspring of irradiated parents. MRR [Internet]. 2013 Jul 1 [cited 2024 Nov 11];753(1):50–67. Available from: https://doi.org/10.1016/j.mrrev.2013.04.001
43. Wang Y, Su M, Chen Y, Huang X, Ruan L, Lv Q, et al. Research progress on the role and mechanism of DNA damage repair in germ cell development. Front Endocrinol [Internet]. 2023 Jul 17 [cited 2024 Nov 11];14:1234280. Available from:
44. PARAMANYA A. Role of oxidative stress in biological systems. MEJS [Internet]. 2019 Dec 29 [cited 2024 Nov 11];5(2):155–62. Available from: https://doi.org/10.23884/mejs.2019.5.2.07
45. Zahra KF, Lefter R, Ali A, Abdellah EC, Trus C, Ciobica A, et al. The Involvement of the Oxidative Stress Status in Cancer Pathology: A Double View on the Role of the Antioxidants. Oxid Med Cell Longev [Internet]. 2021 Jan 1 [cited 2024 Nov 11];2021(1):9965916. Available from: https://onlinelibrary.wiley.com/doi/full/10.1155/2021/9965916
46. Rossnerova A, Izzotti A, Pulliero A, Bast A, Rattan SIS, Rossner P. The Molecular Mechanisms of Adaptive Response Related to Environmental Stress. Int J Mol Sci [Internet]. 2020 Oct 1 [cited 2024 Nov 11];21(19):7053. Available from: https://pmc.ncbi.nlm.nih.gov/articles/PMC7582272/
47. Papaioannou VE, Behringer RR. Phenotypic Analysis of Dominant Mutant Effects in Mice. Cold Spring Harb Protoc [Internet]. 2024 Jan 1 [cited 2024 Nov 11];2024(1). Available from: http://cshprotocols.cshlp.org/content/2024/1/pdb.over107978.full
48. Sánchez-Rodríguez MA, Mendoza-Núñez VM. Oxidative Stress Indexes for Diagnosis of Health or Disease in Humans. Oxid Med Cell Longev [Internet]. 2019 [cited 2024 Nov 11];2019:4128152. Available from: https://pmc.ncbi.nlm.nih.gov/articles/PMC6899293/
49. Iztleuov M, Iztleuov Y, Saparbayev S, Temirbayeva A, Medeuova R, Aleuova Z, et al. Effect of Burdock Root Oil on Oxidative Stress Induced by Isolated and Combined Use of Gamma Radiation and Hexavalent Chromium. Biomed Pharmacol J [Internet]. 2022 Mar 29 [cited 2024 Nov 11];15(1):421–32. Available from: https://dx.doi.org/10.13005/bpj/2382
50. Momo CMM, Ferdinand N, Bebe NKO, Marquise MNA, Augustave K, Narcisse VB, et al. Oxidative Effects of Potassium Dichromate on Biochemical, Hematological Characteristics, and Hormonal Levels in Rabbit Doe (Oryctolagus cuniculus). Vet Sci [Internet]. 2019 Mar 18 [cited 2024 Nov 11];6(1):30. Available from: https://www.mdpi.com/2306-7381/6/1/30/htm
51. Singh V, Singh N, Verma M, Kamal R, Tiwari R, Sanjay Chivate M, et al. Hexavalent-Chromium-Induced Oxidative Stress and the Protective Role of Antioxidants against Cellular Toxicity. Antioxidants [Internet]. 2022 Nov 30 [cited 2024 Nov 11];11(12):2375. Available from: https://www.mdpi.com/2076-3921/11/12/2375/htm
52. Dey S, Nandi A, Das S, Sinha SK, Dey SK. Nicotine and Chromium Co-exposure Lead to Hepatotoxicity in Male Albino Rats. J stress physiol biochem [Internet]. 2023 [cited 2024 Nov 11];19(3). Available from: https://cyberleninka.ru/article/n/nicotine-and-chromium-co-exposure-lead-to-hepatotoxicity-in-male-albino-rats
53. Shyam M, Sabina EP. Harnessing the power of Arctium lappa root: a review of its pharmacological properties and therapeutic applications. Nat Prod Bioprospect [Internet]. 2024 Aug 20 [cited 2024 Nov 11];14(1):1–27. Available from: https://link.springer.com/article/10.1007/s13659-024-00466-8
54. Yosri N, Alsharif SM, Xiao J, Musharraf SG, Zhao C, Saeed A, et al. Arctium lappa (Burdock): Insights from ethnopharmacology potential, chemical constituents, clinical studies, pharmacological utility and nanomedicine. Biomed Pharmacother [Internet]. 2023 Feb 1 [cited 2024 Nov 11];158:114104. Available from: https://doi.org/10.1016/j.biopha.2022.114104
55. Moro TMA, T.P.S. Clerici M. Burdock (Arctium lappa L) roots as a source of inulin-type fructans and other bioactive compounds: Current knowledge and future perspectives for food and non-food applications. Food Res Int [Internet]. 2021 Mar 1 [cited 2024 Nov 11];141:109889. Available from: https://doi.org/10.1016/j.foodres.2020.109889
56. Skowrońska W, Granica S, Dziedzic M, Kurkowiak J, Ziaja M, Bazylko A. Arctium lappa and Arctium tomentosum, Sources of Arctii radix: Comparison of Anti-Lipoxygenase and Antioxidant Activity as well as the Chemical Composition of Extracts from Aerial Parts and from Roots. Plants [Internet]. 2021 Jan 2 [cited 2024 Nov 11];10(1):78. Available from: https://www.mdpi.com/2223-7747/10/1/78/htm
57. Mahboubi M. Arctium Lappa and Management of Liver Functions to Detoxify the Bloodstream. Nat Prod J [Internet]. 2020 Jul 29 [cited 2024 Nov 11];11(5):609–16. Available from: https://doi.org/10.2174/2210315510999200727205254
58. Munir A, Ishrat Fatima, Zelle Humma. Evaluation of Therapeutic Potential of Natural Resources against Breast Cancer. PJBB [Internet]. 2022 Dec 31 [cited 2024 Nov 11];3(2):89-101. Available from: https://pjbb.wum.edu.pk/index.php/ojs/article/view/69
59. Deb A, Gupta S, Mazumder PB. Exosomes: A new horizon in modern medicine. Life Sci [Internet]. 2021 Jan 1 [cited 2024 Nov 11];264:118623. Available from: https://doi.org/10.1016/j.lfs.2020.118623
60. Wu D, Jin L, Huang X, Deng H, Shen Q kun, Quan Z shan, et al. Arctigenin: pharmacology, total synthesis, and progress in structure modification. J Enzyme Inhib Med Chem [Internet]. 2022 [cited 2024 Nov 11];37(1):2452. Available from: https://pmc.ncbi.nlm.nih.gov/articles/PMC9481144/
61. Alhusaini A, Fadda L, Hasan IH, Ali HM, El Orabi NF, Badr AM, et al. Arctium lappa Root Extract Prevents Lead-Induced Liver Injury by Attenuating Oxidative Stress and Inflammation, and Activating Akt/GSK-3β Signaling. Antioxidants [Internet]. 2019 Dec 1 [cited 2024 Nov 11];8(12):582. Available from: https://pmc.ncbi.nlm.nih.gov/articles/PMC6943639/
62. İlgün S, Karatoprak GŞ, Polat DÇ, Şafak EK, Yıldız G, Küpeli Akkol E, et al. Phytochemical Composition and Biological Activities of Arctium minus (Hill) Bernh.: A Potential Candidate as Antioxidant, Enzyme Inhibitor, and Cytotoxic Agent. Antioxidants [Internet]. 2022 Oct 1 [cited 2024 Nov 11];11(10):1852. Available from: https://pmc.ncbi.nlm.nih.gov/articles/PMC9598467/
63. Zhu S, Qiu Z, Qiao X, Waterhouse GIN, Zhu W, Zhao W, et al. Creating burdock polysaccharide-oleanolic acid-ursolic acid nanoparticles to deliver enhanced anti-inflammatory effects: fabrication, structural characterization and property evaluation. Food Sci Hum Wellness [Internet]. 2023 Mar 1 [cited 2024 Nov 11];12(2):454–66. Available from: https://doi.org/10.1016/j.fshw.2022.07.047.
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