Impacts of UC-MSCS on the quality of sperm in asthenoteratozoospermia
DOI:
https://doi.org/10.56294/saludcyt20251104Keywords:
Mesenchymal Stem Cells, secretome, infertility, Asthenoteratozoospermia, Oxidative StressAbstract
Introduction: Male infertility is caused by the condition of male partners, characterized by abnormal sperm shape and decreased motility, called asthenoteratozoospermia. Since active ingredients can regulate biological activity, secretory products from mesenchymal stem cells (MSCs) have recently become objects of interest as drugs.
Methods: The study hypothesis is that UC-MSC secretome impacts sperm quality in asthenoteratozoospermia-affected males depending on the parameters of sperm quality, antioxidant enzyme activity, and ROS concentration. The following sperm quality parameters were assessed, viability, intermotility, ultrastructure, SOD, 8OHdG, and catalase.
Results: Altogether, findings highlight that the stem MSC secretome isolated from the umbilical cord enhances the quality of sperm and will enhance fertility when applied in the process. It also decreases the concentration of biochemical markers of oxidative stress during the sperm preparation process. For example, it leads to a decrease in OHdG and an increase in SOD.
Discussion: The results imply that MSC secretome may be an important therapeutic factor in reproductive health and reduced oxidative stress. According to the data, MSC secretome is likely an anti-oxidative treatment for enhancing the reproductive system
References
Pallotti F, Barbonetti A, Rastrelli G, Santi D, Corona G, Lombardo F. The impact of male factors and their correct and early diagnosis in the infertile couple's pathway: 2021 perspectives. Journal of Endocrinological Investigation. 2022 Oct;45(10):1807-22. https://link.springer.com/article/10.1007/s40618-022-01778-7
2. Barnes A, Riche D, Mena L, Sison T, Barry L, Reddy R, Shwayder J, Parry JP. Efficacy and safety of intrauterine insemination and assisted reproductive technology in populations serodiscordant for human immunodeficiency virus: a systematic review and meta-analysis. Fertility and sterility. 2014 Aug 1;102(2):424-34. https://doi.org/10.1016/j.fertnstert.2014.05.001
3. Ferreux L, Bourdon M, Chargui A, Schmitt A, Stouvenel L, Lorès P, Ray P, Lousqui J, Pocate-Cheriet K, Santulli P, Dulioust E. Genetic diagnosis, sperm phenotype and ICSI outcome in case of severe asthenozoospermia with multiple morphological abnormalities of the flagellum. Human Reproduction. 2021 Nov 1;36(11):2848-60. https://doi.org/10.1093/humrep/deab200
4. Hofer HR, Tuan RS. Secreted trophic factors of mesenchymal stem cells support neurovascular and musculoskeletal therapies. Stem cell research & therapy. 2016 Dec;7:1-4. https://doi.org/10.1186/s13287-016-0394-0
5. Fadilah NI, Jailani MS, Hisham MA, Raj NS, Shamsuddin SA, Ng MH, Fauzi MB, Maarof M. Cell secretomes for wound healing and tissue regeneration: Next generation acellular based tissue engineered products. Journal of Tissue Engineering. 2022 Jan;13. 10.1177/20417314221114273
6. Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, Squadrito F, Altavilla D, Bitto A. Oxidative stress: harms and benefits for human health. Oxidative medicine and cellular longevity. 2017;2017(1):8416763. https://doi.org/10.1155/2017/8416763
7. Banche Niclot AG, Marini E, Ferrero I, Barbero F, Rosso E, Fenoglio I, Barge A, Pessina A, Coccè V, Paino F, Mareschi K. A New Paclitaxel Formulation Based on Secretome Isolated from Mesenchymal Stem Cells Shows a Significant Cytotoxic Effect on Osteosarcoma Cell Lines. Pharmaceutics. 2023 Sep 19;15(9):2340. https://doi.org/10.3390/pharmaceutics15092340
8. Kalinina N, Kharlampieva D, Loguinova M, Butenko I, Pobeguts O, Efimenko A, Ageeva L, Sharonov G, Ischenko D, Alekseev D, Grigorieva O. Characterization of secretomes provides evidence for adipose-derived mesenchymal stromal cells subtypes. Stem cell research & therapy. 2015 Dec;6:1-2. https://doi.org/10.1186/s13287-015-0209-8
9. Idrisova KF, Zeinalova AK, Masgutova GA, Bogov Jr AA, Allegrucci C, Syromiatnikova VY, Salafutdinov II, Garanina EE, Andreeva DI, Kadyrov AA, Rizvanov AA. Application of neurotrophic and proangiogenic factors as therapy after peripheral nervous system injury. Neural Regeneration Research. 2022 Jun 1;17(6):1240-7. https://journals.lww.com/nrronline/fulltext/2022/06000/Application_of_neurotrophic_and_proangiogenic.10.aspx
10. Kaltsas A. Oxidative stress and male infertility: the protective role of anti-oxidants. Medicina. 2023 Oct 4;59(10):1769. https://doi.org/10.3390/medicina59101769
11. Pasciu V, Nieddu M, Sotgiu FD, Baralla E, Berlinguer F. An Overview on Assay Methods to Quantify ROS and Enzymatic Antioxidants in Erythrocytes and Spermatozoa of Small Domestic Ruminants. Animals. 2023 Jul 13;13(14):2300. https://doi.org/10.3390/ani13142300
12. Bader R, Ibrahim JN, Mourad A, Moussa M, Azoury J, Azoury J, Alaaeddine N. Improvement of human sperm vacuolization and DNA fragmentation co-cultured with adipose-derived mesenchymal stem cell secretome: in vitro effect. International journal of stem cells. 2019 Nov 30;12(3):388-99. DOI: https://doi.org/10.15283/ijsc19047
13. Xu L, Yang J, Xu M, Shan D, Wu Z, Yuan D. Speciation and adaptive evolution reshape anti-oxidant enzymatic system diversity across the phylum Nematoda. BMC biology. 2020 Dec;18:1-8. https://doi.org/10.1186/s12915-020-00896-z
14. Singh AK, Kumar A, Bisla A. Computer-assisted sperm analysis (CASA) in veterinary science: A review. The Indian Journal of Animal Sciences. 2021 Jun 1;91(6):419-29. https://doi.org/10.56093/ijans.v91i6.115435
15. Ahmad F, Khan RA. A power comparison of various normality tests. Pakistan Journal of Statistics and Operation Research. 2015 Sep 8:331-45. https://doi.org/10.18187/pjsor.v11i3.845
16. Colpi GM, Francavilla S, Haidl G, Link K, Behre HM, Goulis DG, Krausz C, Giwercman A. European Academy of Andrology guideline Management of oligo‐astheno‐teratozoospermia. Andrology. 2018 Jul;6(4):513-24. https://doi.org/10.1111/andr.12502
17. Hasan MM, Kabir MF, Khandaker MA, Kamruzzaman AH, Akter M, Nuri A. Management of Severe Asthenoteratozoospermia with Traditional Medicine: A Case Study. European Journal of Medical and Health Research. 2024 Jan 1;2(1):91-4. https://doi.org/10.59324/ejmhr.2024.2(1).13
18. Chemes HE, Rawe VY. Sperm pathology: a step beyond descriptive morphology. Origin, characterization and fertility potential of abnormal sperm phenotypes in infertile men. Human Reproduction Update. 2003 Sep 1;9(5):405-28. https://doi.org/10.1093/humupd/dmg034
19. EJACULATION WT. Prasad V Kulkarni1, Krushndev L Shende2. Int. J. Ayur. Pharma Research. 2013;1(3):47-53. https://core.ac.uk/download/pdf/333819227.pdf
20. Zi T, Liu Y, Zhang Y, Wang Z, Wang Z, Zhan S, Peng Z, Li N, Liu X, Liu F. Protective effect of melatonin on alleviating early oxidative stress induced by DOX in mice spermatogenesis and sperm quality maintaining. Reproductive Biology and Endocrinology. 2022 Jul 18;20(1):105. https://doi.org/10.1186/s12958-022-00977-4
21. Ghafouri-Fard S, Shoorei H, Abak A, Seify M, Mohaqiq M, Keshmir F, Taheri M, Ayatollahi SA. Effects of chemotherapeutic agents on male germ cells and possible ameliorating impact of anti-oxidants. Biomedicine & Pharmacotherapy. 2021 Oct 1;142:112040. https://doi.org/10.1016/j.biopha.2021.112040
22. Wang Y, Zhou Y, Zheng Z, Li J, Yan Y, Wu W. Sulforaphane metabolites reduce resistance to paclitaxel via microtubule disruption. Cell death & disease. 2018 Nov 14;9(11):1134. https://doi.org/10.1038/s41419-018-1174-9
23. González-González A, García-Sánchez D, Dotta M, Rodríguez-Rey JC, Pérez-Campo FM. Mesenchymal stem cells secretome: The cornerstone of cell-free regenerative medicine. World journal of stem cells. 2020 Dec 12;12(12):1529. https://doi.org/10.4252/wjsc.v12.i12.1529
24. Bari E, Perteghella S, Di Silvestre D, Sorlini M, Catenacci L, Sorrenti M, Marrubini G, Rossi R, Tripodo G, Mauri P, Marazzi M. Pilot production of mesenchymal stem/stromal freeze-dried secretome for cell-free regenerative nanomedicine: a validated GMP-compliant process. Cells. 2018 Oct 30;7(11):190. https://doi.org/10.3390/cells7110190
25. Panahi M, Rahimi B, Rahimi G, Yew Low T, Saraygord‐Afshari N, Alizadeh E. Cytoprotective effects of anti-oxidant supplementation on mesenchymal stem cell therapy. Journal of cellular physiology. 2020 Oct;235(10):6462-95. https://doi.org/10.1002/jcp.29660
26. Ajayi AF, Akhigbe RE. The physiology of male reproduction: Impact of drugs and their abuse on male fertility. Andrologia. 2020 Oct;52(9):e13672. https://doi.org/10.1111/and.13672
27. Wang Z, Teng Z, Wang Z, Song Z, Zhu P, Li N, Zhang Y, Liu X, Liu F. Melatonin ameliorates paclitaxel‐induced mice spermatogenesis and fertility defects. Journal of Cellular and Molecular Medicine. 2022 Feb;26(4):1219-28. https://doi.org/10.1111/jcmm.17177
28. Qian C, Meng Q, Lu J, Zhang L, Li H, Huang B. Human amnion mesenchymal stem cells restore spermatogenesis in mice with busulfan-induced testis toxicity by inhibiting apoptosis and oxidative stress. Stem cell research & therapy. 2020 Dec;11:1-2. https://doi.org/10.1186/s13287-020-01803-7
29. Ghasemzadeh-Hasankolaei M, Batavani R, Eslaminejad MB, Sayahpour F. Transplantation of autologous bone marrow mesenchymal stem cells into the testes of infertile male rats and new germ cell formation. International journal of stem cells. 2016 Nov 30;9(2):250-63. DOI: https://doi.org/10.15283/ijsc16010
30. Zhang Y, Liu Y, Teng Z, Wang Z, Zhu P, Wang Z, Liu F, Liu X. Human umbilical cord mesenchymal stem cells (hUC-MSCs) alleviate paclitaxel-induced spermatogenesis defects and maintain male fertility. Biological Research. 2023 Aug 13;56(1):47. DOI:10.1186/s40659-023-00459-w
31. Merzenich H, Zeeb H, Blettner M. Decreasing sperm quality: a global problem?. BMC public health. 2010 Dec;10:1-5. https://doi.org/10.1186/1471-2458-10-24
32. Yang RF, Liu TH, Zhao K, Xiong CL. Enhance mouse germ cell-associated gene expression by injecting human umbilical cord mesenchymal stem cells into the testis of chemical-induced azoospermic mice. Asian Journal of Andrology. 2014 Sep 1;16(5):698-704. DOI: 10.4103/1008-682X.129209
33. Ahmed EA, Ahmed OM, Fahim HI, Mahdi EA, Ali TM, Elesawy BH, Ashour MB. Combinatory Effects of Bone Marrow‐Derived Mesenchymal Stem Cells and Indomethacin on Adjuvant‐Induced Arthritis in Wistar Rats: Roles of IL‐1β, IL‐4, Nrf‐2, and Oxidative Stress. Evidence‐Based Complementary and Alternative Medicine. 2021;2021(1):8899143. https://doi.org/10.1155/2021/8899143
34. Milardi D, Grande G, Sacchini D, Astorri AL, Pompa G, Giampietro A, De Marinis L, Pontecorvi A, Spagnolo AG, Marana R. Male fertility and reduction in semen parameters: a single tertiary‐care center experience. International journal of endocrinology. 2012;2012(1):649149. https://doi.org/10.1155/2012/649149
35. Sagaradze G, Monakova A, Efimenko A. Potency Assays for Mesenchymal Stromal Cell Secretome-Based Products for Tissue Regeneration. International Journal of Molecular Sciences. 2023 May 27;24(11):9379. https://doi.org/10.3390/ijms24119379
36. Chen S, He Z, Xu J. Application of adipose-derived stem cells in photoaging: basic science and literature review. Stem Cell Research & Therapy. 2020 Dec;11:1-5. https://doi.org/10.1186/s13287-020-01994-z
37. LEWIS B, AITKEN RJ. A redox‐regulated tyrosine phosphorylation cascade in rat spermatozoa. Journal of Andrology. 2001 Jul 8;22(4):611-22. https://doi.org/10.1002/j.1939-4640.2001.tb02221.x.
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Ria Margiana, Rajendra Falah Adriansyah, Mohammad Ayodhia Soebadi, Reny I’tishom, Tania Graciana, Silvia W. Lestari (Author)
This work is licensed under a Creative Commons Attribution 4.0 International License.
The article is distributed under the Creative Commons Attribution 4.0 License. Unless otherwise stated, associated published material is distributed under the same licence.
